4HOT
Crystal Structure of Full-Length Human IFIT5 with 5`-triphosphate Oligoadenine
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2012-07-15 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.627, 85.188, 60.651 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 34.431 - 2.501 |
| R-factor | 0.1845 |
| Rwork | 0.180 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.017 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 18505 | |
| Completeness [%] | 97.9 | 82.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295.15 | -, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
