4HNF
Crystal structure of ck1d in complex with pf4800567
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 171.217, 48.109, 80.467 |
Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.070 |
Rwork | 0.241 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.078 | 0.591 |
Number of reflections | 37338 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100 mM Citrate, 1.0 M LiCl, 20-30% PEG6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |