4HKZ

Trastuzumab Fab complexed with Protein L and Protein A fragments

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837
Spacegroup name	P 21 21 21
Unit cell lengths	53.520, 104.670, 117.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.810 - 2.080
R-factor	0.1643
Rwork	0.162
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.066
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.810	2.130
High resolution limit [Å]	2.080	2.080
Rmerge	0.067	0.395
Number of reflections	40077
<I/σ(I)>	14.4	3.1
Completeness [%]	99.0	92.3
Redundancy	4.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	50 mM Hepes, 20% PEG 3350, 100 mM NaCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K