4HKC
14-3-3-zeta in complex with S1011 phosphorylated integrin alpha-4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2011-01-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9163 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.030, 111.600, 72.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.800 - 2.200 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o02 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.394 | |
| Number of reflections | 18798 | |
| <I/σ(I)> | 9.8 | 3.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M Na HEPES (pH 7.5), 25% PEG 2000 MME, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
