4HKC
14-3-3-zeta in complex with S1011 phosphorylated integrin alpha-4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2011-01-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9163 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.030, 111.600, 72.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.800 - 2.200 |
R-factor | 0.1991 |
Rwork | 0.197 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o02 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.394 | |
Number of reflections | 18798 | |
<I/σ(I)> | 9.8 | 3.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M Na HEPES (pH 7.5), 25% PEG 2000 MME, VAPOR DIFFUSION, SITTING DROP, temperature 277K |