4HHO
Serum paraoxonase-1 by directed evolution with the H115W mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 93.782, 93.782, 145.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.890 - 2.100 |
R-factor | 0.18449 |
Rwork | 0.183 |
R-free | 0.21243 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.029 |
RMSD bond angle | 2.335 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.890 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.071 | 0.574 |
Number of reflections | 36561 | |
<I/σ(I)> | 26.12 | 2.97 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 20% PEG 3350, 0.2M NaBr, 0.1M Bis-tris propane , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |