4HHM

Crystal structure of a mutant, G219A, of Glucose Isomerase from Streptomyces sp. SK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-11
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	85.500, 137.400, 136.600
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	48.500 - 2.150
R-factor	0.197
Rwork	0.197
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1muw
RMSD bond length	0.007
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.200
High resolution limit [Å]	2.150	2.150
Number of reflections	163122
<I/σ(I)>	11.7
Completeness [%]	95.4	86.3
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	PEG 3350, Mg acetate, pH 7, VAPOR DIFFUSION, temperature 298.0K