4HHH

Structure of Pisum sativum Rubisco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-10
Detector	Marmosaic
Wavelength(s)	0.98
Spacegroup name	P 21 2 21
Unit cell lengths	109.790, 109.950, 201.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.950 - 2.200
R-factor	0.2014
Rwork	0.197
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	2.446
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHENIX (MR)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	109.950	48.201	2.320
High resolution limit [Å]	2.200	6.960	2.200
Rmerge		0.147	0.387
Total number of observations		10088	44423
Number of reflections	116075
<I/σ(I)>	3.8	3.1	1.7
Completeness [%]	94.1	89.1	95.5
Redundancy	2.7	2.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	10% PEG6000, 0.1 M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 293K