4HHH
Structure of Pisum sativum Rubisco
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-10 |
Detector | Marmosaic |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 109.790, 109.950, 201.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 109.950 - 2.200 |
R-factor | 0.2014 |
Rwork | 0.197 |
R-free | 0.27940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 2.446 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHENIX (MR) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 109.950 | 48.201 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.147 | 0.387 | |
Total number of observations | 10088 | 44423 | |
Number of reflections | 116075 | ||
<I/σ(I)> | 3.8 | 3.1 | 1.7 |
Completeness [%] | 94.1 | 89.1 | 95.5 |
Redundancy | 2.7 | 2.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% PEG6000, 0.1 M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 293K |