4HFM
X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 88.200, 149.840, 66.610 |
Unit cell angles | 90.00, 115.53, 90.00 |
Refinement procedure
Resolution | 57.830 - 1.900 |
R-factor | 0.17456 |
Rwork | 0.173 |
R-free | 0.20553 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3J3H |
RMSD bond length | 0.016 |
RMSD bond angle | 1.612 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 60.110 |
High resolution limit [Å] | 1.900 |
Number of reflections | 59835 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100mM KH2PO4/citrate buffer pH 4.5 + 45% PEG 300, VAPOR DIFFUSION, SITTING DROP, temperature 293K |