4HFH
The GLIC pentameric Ligand-Gated Ion Channel (wild-type) complexed to bromoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2011-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.000, 132.650, 159.270 |
| Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
| Resolution | 21.710 - 2.650 |
| R-factor | 0.2025 |
| Rwork | 0.202 |
| R-free | 0.21340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.082 | 0.821 |
| Number of reflections | 105994 | |
| <I/σ(I)> | 15 | 2.1 |
| Completeness [%] | 99.1 | 96.1 |
| Redundancy | 8.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 2% bromoform, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
