4HFB
The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant (Apo)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9801 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 183.737, 133.512, 161.120 |
Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.750 |
R-factor | 0.2085 |
Rwork | 0.208 |
R-free | 0.22230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.600 | 48.630 | 2.900 |
High resolution limit [Å] | 2.750 | 8.690 | 2.750 |
Rmerge | 0.044 | 0.018 | 0.630 |
Number of reflections | 97187 | ||
<I/σ(I)> | 15.6 | 50.6 | 2 |
Completeness [%] | 98.8 | 97.3 | 96.7 |
Redundancy | 3.4 | 3.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 12-15% PEG4K, 0.1M Na Acetate 0.4M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 291K |