4HF2
Crystal Structure of E43A IscR mutant bound to its promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-13 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.622, 75.184, 186.448 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.110 - 2.990 |
| R-factor | 0.233 |
| Rwork | 0.229 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.017 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (dev_1125) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.560 | |
| Number of reflections | 14274 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 98.8 | 95 |
| Redundancy | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 2M Ammonium Sulfate, 20% glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
