4HBG
Crystal structure of Saccharomyces cerevisiae 3 oxoacyl-[acyl carrier protein]-reductase complexed with NADPH (form2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.800, 50.800, 415.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.930 - 2.270 |
| R-factor | 0.24674 |
| Rwork | 0.245 |
| R-free | 0.28502 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.270 |
| Rmerge | 0.066 |
| Number of reflections | 14440 |
| Completeness [%] | 94.0 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 30% V/V JEFFAMINE ED2001, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
