4HAN

Crystal structure of Galectin 8 with NDP52 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 31 1 2
Unit cell lengths	71.884, 71.884, 314.493
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.096 - 2.551
R-factor	0.1928
Rwork	0.191
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.532
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.100
High resolution limit [Å]	2.550
Number of reflections	30888
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.1M Tris-Hcl pH8.5, 30~34% (v/w) PEG400, 200mM LiSO4, and 10mM Nicotinamide adenine dinucleotide, VAPOR DIFFUSION, HANGING DROP, temperature 295K