4HAK
Multicopper Oxidase CueO mutant E506A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.439, 90.867, 53.244 |
| Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
| Resolution | 30.200 - 1.400 |
| R-factor | 0.16047 |
| Rwork | 0.159 |
| R-free | 0.18378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.387 |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 91858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 100mM Acetate-Na pH5.0, 22% PEG 4000, 5% Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
