4H8S
Crystal structure of human APPL2BARPH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953698 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.960, 208.013, 218.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.950 - 3.500 |
| R-factor | 0.2116 |
| Rwork | 0.209 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q13 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.976 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1031)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.950 | |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.175 | |
| Number of reflections | 55792 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 93.9 | |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.9% w/v PEG8000, 40 mM hexamine cobalt(III) chloride, 20% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
