4H8I
Structure of GluK2-LBD in complex with GluAzo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.609, 102.609, 282.513 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 2.000 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g3f |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.070 | 0.014 | 0.605 |
| Number of reflections | 60248 | 512 | 8042 |
| <I/σ(I)> | 27.54 | 94.98 | 3.98 |
| Completeness [%] | 99.8 | 84.5 | 100 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.05 M lithium sulfate, 10 mM magnesium chloride, 50 mM MES sodium, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
