4H5M
Crystal Structure of Rift Valley Fever Virus Nucleocapsid Protein Hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0332 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 107.130, 107.130, 258.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.390 - 3.100 |
R-factor | 0.2175 |
Rwork | 0.215 |
R-free | 0.25580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lyf |
RMSD bond length | 0.009 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 92.777 | 46.389 | 3.270 |
High resolution limit [Å] | 3.100 | 9.800 | 3.100 |
Rmerge | 0.025 | 0.693 | |
Total number of observations | 7175 | 20670 | |
Number of reflections | 31959 | ||
<I/σ(I)> | 13.1 | 20.7 | 1.1 |
Completeness [%] | 91.9 | 98.9 | 89.7 |
Redundancy | 6.2 | 6.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 24% PEG3350, 350 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |