4H5M

Crystal Structure of Rift Valley Fever Virus Nucleocapsid Protein Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 31 2 1
Unit cell lengths	107.130, 107.130, 258.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.390 - 3.100
R-factor	0.2175
Rwork	0.215
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lyf
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.777	46.389	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge		0.025	0.693
Total number of observations		7175	20670
Number of reflections	31959
<I/σ(I)>	13.1	20.7	1.1
Completeness [%]	91.9	98.9	89.7
Redundancy	6.2	6.3	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% PEG3350, 350 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K