4H3Q
Crystal structure of human ERK2 complexed with a MAPK docking peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-31 |
Detector | PSI PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.750, 58.530, 159.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.149 - 2.200 |
R-factor | 0.1839 |
Rwork | 0.181 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tei |
RMSD bond length | 0.010 |
RMSD bond angle | 1.415 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.150 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 20375 | |
Completeness [%] | 99.0 | 96.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 20% PEG1500, 0.1M MIB, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |