4H26
TCR interaction with peptide mimics of nickel offers structure insight to nickel contact allergy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.822, 129.202, 97.473 |
Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.225 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 30552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 16% PEG4000, 100mM Tris-HCL, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |