4H22
Crystal structure of the dimeric coiled-coil domain of the cytosolic nucleic acid sensor LRRFIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.363, 71.447, 68.035 |
| Unit cell angles | 90.00, 110.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.890 |
| R-factor | 0.23529 |
| Rwork | 0.235 |
| R-free | 0.24757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q6q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.940 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 15465 | |
| Completeness [%] | 99.7 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG 8000, MES pH 6.5, sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
