4GZN

Mouse ZFP57 zinc fingers in complex with methylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	40.218, 60.641, 96.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.683 - 0.990
R-factor	0.1319
Rwork	0.131
R-free	0.13960
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.799
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.600	1.070	1.030
High resolution limit [Å]	0.990	1.030	0.990
Rmerge	0.133	0.329	0.404
Number of reflections	802194
<I/σ(I)>	16.1		2.8
Completeness [%]	94.4	85.3	58.9
Redundancy	12.9	7.6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	25% MPD, 15% PEG8000, 100mM CaCl2 and 100 mM acetate pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 289K