4GYZ

Mus Musculus Tdp2 Bound to dAMP and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	85.347, 169.326, 184.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.977 - 2.556
R-factor	0.1927
Rwork	0.191
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Rmerge	0.105	0.064	0.510
Number of reflections	82947
<I/σ(I)>	13.5
Completeness [%]	97.7	97.7	92.1
Redundancy	5.3	5.8	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	PEG 6000, 10mM dAMP, 100mM MES, 10mM MgCl2, pH 6.0, VAPOR DIFFUSION, temperature 293K