4GYB

Two-domain laccase from streptomyces lividans at 2.4 A resolution AC1709

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-08
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 43 3 2
Unit cell lengths	177.540, 177.540, 177.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.385 - 2.400
R-factor	0.1548
Rwork	0.154
R-free	0.17710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kw8
RMSD bond length	0.007
RMSD bond angle	1.119
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	37874
<I/σ(I)>	11.8
Completeness [%]	99.9	99.7
Redundancy	8.68	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	296	31%(v/v) PEG monomethyl ether 550, 0.1M NaCl, 0.1M glycine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 296K