3KW8
Two-domain laccase from Streptomyces coelicolor at 2.3 A resolution
Summary for 3KW8
| Entry DOI | 10.2210/pdb3kw8/pdb |
| Related | 3CG8 |
| Descriptor | Putative copper oxidase, COPPER (II) ION, FE (III) ION, ... (7 entities in total) |
| Functional Keywords | two-domain laccase, oxidoreductase, multicopper blue protein |
| Biological source | Streptomyces coelicolor |
| Total number of polymer chains | 1 |
| Total formula weight | 31573.84 |
| Authors | Skalova, T.,Dohnalek, J.,Kolenko, P.,Duskova, J.,Stepankova, A.,Hasek, J.,Ostergaard, L.H.,Ostergaard, P.R. (deposition date: 2009-12-01, release date: 2010-12-08, Last modification date: 2023-11-01) |
| Primary citation | Skalova, T.,Duskova, J.,Hasek, J.,Stepankova, A.,Koval, T.,Ostergaard, L.H.,Dohnalek, J. Structure of laccase from Streptomyces coelicolor after soaking with potassium hexacyanoferrate and at an improved resolution of 2.3 A Acta Crystallogr.,Sect.F, 67:27-32, 2011 Cited by PubMed Abstract: The paper reports the structure of the small laccase from Streptomyces coelicolor determined from a crystal soaked with potassium hexacyanoferrate [K4Fe(CN)6]. The decolorization of the natively blue crystal observed upon soaking indicates the reduction of the enzyme in the crystal. The ligand binds between laccase molecules and stabilizes the crystal. The increased diffraction limit of the diffraction data collected from this crystal enabled the refinement of the small laccase structure at 2.3 Å resolution, which is the highest resolution obtained to date. PubMed: 21206017DOI: 10.1107/S1744309110046099 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.29 Å) |
Structure validation
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