4GXY
RNA structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.1049 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 96.633, 96.633, 132.684 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.347 - 3.050 |
| R-factor | 0.2102 |
| Rwork | 0.208 |
| R-free | 0.25030 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.189 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.201 | 0.660 |
| Number of reflections | 13827 | |
| <I/σ(I)> | 26.6 | 2.6 |
| Completeness [%] | 97.8 | 99.1 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 240 mM sodium malonate, 22 % PEG3350 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
