4GXU
Crystal structure of antibody 1F1 bound to the 1918 influenza hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2009-01-09 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 153.711, 176.269, 168.120 |
Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
Resolution | 35.036 - 3.294 |
R-factor | 0.2206 |
Rwork | 0.219 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gxv |
RMSD bond length | 0.005 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.294 | 3.294 |
Number of reflections | 108722 | |
<I/σ(I)> | 14.2 | 1.9 |
Completeness [%] | 80.6 | 34.9 |
Redundancy | 3.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 8.5% PEG6000, 100 mM Tris, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K |