4GWG

Crystal Structure Analysis of 6-phosphogluconate dehydrogenase apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	180
Detector technology	CCD
Collection date	2011-12-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 43 21 2
Unit cell lengths	87.280, 87.280, 130.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.091 - 1.391
R-factor	0.1696
Rwork	0.169
R-free	0.18570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fwn
RMSD bond length	0.005
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.391
Number of reflections	99063
Completeness [%]	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	14% PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K