4GV9

Lassa nucleoprotein C-terminal domain in complex with triphosphated dsRNA soaking for 5 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	173
Detector technology	CCD
Collection date	2011-06-06
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9763
Spacegroup name	P 41 21 2
Unit cell lengths	44.780, 44.780, 221.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.890 - 2.460
R-factor	0.261
Rwork	0.259
R-free	0.30466
RMSD bond length	0.004
RMSD bond angle	0.911
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.900	2.600
High resolution limit [Å]	2.460	2.460
Rmerge	0.058	0.785
Number of reflections	8461
<I/σ(I)>	2.4	5.6
Completeness [%]	98.4	99
Redundancy	5.8	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	18.5% PEG MME 5000, 0.1 M sodium citrate (pH4.5), and 2.4% PEG MME350, VAPOR DIFFUSION, SITTING DROP, temperature 293K