4GV8
DUTPase from phage phi11 of S.aureus: visualization of the species-specific insert
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-30 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 109.660, 109.660, 169.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.100 - 2.100 |
R-factor | 0.1858 |
Rwork | 0.183 |
R-free | 0.23224 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2py4 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.888 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.104 | 47.100 | 2.150 |
High resolution limit [Å] | 2.100 | 2.150 | 2.100 |
Rmerge | 0.058 | 0.978 | |
Number of reflections | 60917 | ||
<I/σ(I)> | 34.37 | 3.07 | |
Redundancy | 13.3 | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 25% MPD, 0.02M calcium chloride, 0.1M sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |