4GST

REACTION COORDINATE MOTION IN AN SNAR REACTION CATALYZED BY GLUTATHIONE TRANSFERASE

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.9 Å)

Cell axes88.24069.44081.280
Cell angles90.00106.0190.00
SpacegroupC 1 2 1
Resolution limits - 1.90
the highest resolution shell value -
R-factor0.18 (0.180*)
RMSD bond length0.017
RMSD bond angle0.040*

Data Collection Statistics

Resolution limits - 1.90*
the highest resolution shell value -
Number of reflections36138*
Rmerge_l_obs0.066*

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein11.3 (mg/ml)
21drop(9R,-10R)-9,10-dihydro-9-(S-glutathionyl)-10-hydroxyphenanthrene3.5 (mM)
31dropbeta-octylglucopyranoside0.4 (%(w/v))
41dropammonium sulfate40 (%(w/v)sat)
51drop10 (mM)
61reservoirammonium sulfate60-74 (%(w/v)sat)
71reservoirbeta-octylglucopyranoside0.46 (%(w/v))
81reservoir10 (mM)
Annotated Information is extracted from Literature Info*
'Sesay, M. A.', (1987) J.Mol.Biol., 197, 377.