4GRU
crystallographic and biological characterization of N- and C- terminus mutants of human MIF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.562, 95.562, 103.778 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.2009 |
| Rwork | 0.201 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human MIF |
| RMSD bond length | 1.230 |
| RMSD bond angle | 0.005 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.920 |
| Rmerge | 0.096 |
| Number of reflections | 42321 |
| <I/σ(I)> | 22.6 |
| Completeness [%] | 99.8 |
| Redundancy | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0M ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
