4GRQ
Characterization of N- and C- terminus mutants of human MIF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-10 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 95.369, 95.369, 103.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.680 - 1.650 |
| R-factor | 0.139 |
| Rwork | 0.137 |
| R-free | 0.16100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MIF |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.748 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.720 |
| High resolution limit [Å] | 1.470 | 1.650 |
| Rmerge | 0.085 | 0.555 |
| Number of reflections | 93378 | |
| <I/σ(I)> | 26.7 | 5.4 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 11.2 | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 310 | 1.6M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
