4GPU
Crystal structure of K. lactis Dxo1 (YDR370C) in complex with manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 82.130, 82.130, 260.730 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.850 - 2.800 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.25700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4gps |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 13599 |
Completeness [%] | 99.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M Sodium citrate tribasic, 23.5% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |