4GOS
Crystal structure of human B7-H4 IgV-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750, 1.5402 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 46.500, 46.500, 115.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 1.590 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.19160 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.474 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHELX |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.149 | 38.590 | 1.680 |
High resolution limit [Å] | 1.590 | 5.030 | 1.590 |
Total number of observations | 8555 | 34525 | |
Number of reflections | 16143 | ||
<I/σ(I)> | 17.7 | 13.9 | 1.6 |
Completeness [%] | 90.1 | 98.9 | 100 |
Redundancy | 13.3 | 12.7 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 291 | 200 mM tri-Potassium citrate, 2.2 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, temperature 291K |
2 | VAPOR DIFFUSION | 7 | 291 | 200 mM tri-Potassium citrate, 2.2 M ammonium sulfate, I3C (magic triangle) soak, pH 7.0, VAPOR DIFFUSION, temperature 291K |