4GO2
Crystal structure of the c-terminal domain of 10'formyltetrahydrofolate dehydrogenase in complex with Thio-NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 258.935, 194.391, 97.114 |
| Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
| Resolution | 49.880 - 2.280 |
| R-factor | 0.17791 |
| Rwork | 0.177 |
| R-free | 0.19634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o2p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.096 | 0.560 |
| Number of reflections | 205602 | |
| <I/σ(I)> | 11.7 | 1.9 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 1.6 M Ammonium Sulfate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
