4GNE
Crystal Structure of NSD3 tandem PHD5-C5HCH domains complexed with H3 peptide 1-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 57.200, 57.200, 107.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.420 - 1.470 |
| R-factor | 0.1495 |
| Rwork | 0.148 |
| R-free | 0.17850 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.297 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.536 | 36.417 | 1.540 |
| High resolution limit [Å] | 1.465 | 4.630 | 1.470 |
| Rmerge | 0.066 | 0.281 | |
| Rmeas | 0.076 | 0.293 | |
| Rpim | 0.013 | 0.047 | |
| Total number of observations | 22729 | 96987 | |
| Number of reflections | 18490 | ||
| <I/σ(I)> | 36.7 | 51.1 | 15.4 |
| Completeness [%] | 99.5 | 96.9 | 98.5 |
| Redundancy | 39.7 | 33.1 | 37.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 0.1M Tris-HCl pH 7.0, 30% (w/v) PEG 3000, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
