4GND

Crystal Structure of NSD3 tandem PHD5-C5HCH domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.278
Spacegroup name	P 65
Unit cell lengths	58.510, 58.510, 108.581
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.670 - 2.270
R-factor	0.1751
Rwork	0.172
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gne
RMSD bond length	0.007
RMSD bond angle	1.195
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.671	50.671	2.390
High resolution limit [Å]	2.270	7.180	2.270
Rmerge		0.054	0.308
Rmeas		0.055	0.317
Rpim		0.012	0.074
Total number of observations		6730	25287
Number of reflections	9797
<I/σ(I)>	28	47.1	10.5
Completeness [%]	100.0	99.9	100
Redundancy	19	20.4	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	283	0.1M Hepes, 1.1M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K