4GN5
OBody AM3L15 bound to hen egg-white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-17 |
| Detector | MAR 2300 |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.990, 58.830, 95.180 |
| Unit cell angles | 90.00, 95.43, 90.00 |
Refinement procedure
| Resolution | 31.150 - 1.860 |
| R-factor | 0.1747 |
| Rwork | 0.173 |
| R-free | 0.20240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.863 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 94.753 | 31.150 | 1.960 |
| High resolution limit [Å] | 1.860 | 5.880 | 1.860 |
| Rmerge | 0.042 | 0.533 | |
| Total number of observations | 4755 | 17264 | |
| Number of reflections | 46187 | ||
| <I/σ(I)> | 8.1 | 12.4 | 1.4 |
| Completeness [%] | 95.7 | 92.7 | 92.3 |
| Redundancy | 2.8 | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 0.2 M HEPES, 5% MPEG5000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
