4GMH
Crystal structure of staphylococcus aureus 5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-07-17 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 47.423, 55.852, 97.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.720 - 2.000 |
| R-factor | 0.18591 |
| Rwork | 0.183 |
| R-free | 0.23305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | S AUREUS MTAN 3BL6 |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.117 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.930 | 2.110 |
| High resolution limit [Å] | 1.750 | 2.000 |
| Rmerge | 0.150 | 0.660 |
| Number of reflections | 18068 | |
| <I/σ(I)> | 3.49 | 1.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.91 | 6.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 4.2M FORMATE, 0.1M TRIS HCL, CRYOPROTECTANT 20% GLYCEROL, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 110K |
