4GLV

OBody AM3L09 bound to hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-16
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95666
Spacegroup name	P 1
Unit cell lengths	59.000, 69.380, 76.360
Unit cell angles	72.17, 69.46, 77.55

Refinement procedure

Resolution	44.826 - 2.574
R-factor	0.2036
Rwork	0.201
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.984
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.196	48.009	2.710
High resolution limit [Å]	2.574	8.140	2.574
Rmerge		0.061	0.422
Total number of observations		7802	35598
Number of reflections	32670
<I/σ(I)>	14	7.4	1.6
Completeness [%]	96.2	94.9	93.6
Redundancy	7.8	7.6	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	291	0.2 M HEPES, 7% MPEG5000, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 291K