4GKS
A2-MHC Complex carrying FLTGIGIITV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.91730 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.540, 46.780, 115.860 |
| Unit cell angles | 90.00, 121.66, 90.00 |
Refinement procedure
| Resolution | 39.049 - 2.346 |
| R-factor | 0.215 |
| Rwork | 0.211 |
| R-free | 0.28520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gkn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.664 |
| Data reduction software | xia2 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.049 | 39.049 | 2.410 |
| High resolution limit [Å] | 2.346 | 10.490 | 2.346 |
| Rmerge | 0.046 | 0.701 | |
| Total number of observations | 1578 | 10780 | |
| Number of reflections | 36005 | ||
| <I/σ(I)> | 8.8 | 12.4 | 1 |
| Completeness [%] | 92.4 | 92.1 | 94.2 |
| Redundancy | 4 | 3.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG 8000, 200 mM Ammonium Sulphate, 200 mM Cacodylate Buffer, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
