4GIL

Crystal Structure of Pseudouridine Monophosphate Glycosidase/Linear Pseudouridine 5'-Phosphate Adduct

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	61.472, 77.116, 200.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.069 - 2.539
R-factor	0.1924
Rwork	0.189
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vkm
RMSD bond length	0.008
RMSD bond angle	1.267
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.630
High resolution limit [Å]	2.539	5.470	2.539
Rmerge	0.081	0.040	0.478
Number of reflections	32156
<I/σ(I)>	10.6
Completeness [%]	99.3	99.8	97.9
Redundancy	3.9	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	PEG4000, Tris, sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K