4GIK

Crystal Structure of Pseudouridine Monophosphate Glycosidase/Linear R5P Adduct

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	61.347, 116.496, 132.153
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.204 - 2.187
R-factor	0.1819
Rwork	0.179
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vkm
RMSD bond length	0.007
RMSD bond angle	1.178
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.187	4.740	2.187
Rmerge	0.082	0.030	0.470
Number of reflections	49152
<I/σ(I)>	7.4
Completeness [%]	99.8	98.9	99.9
Redundancy	4	3.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	22% PEG4000, 0.1 M Tris, 0.2 M sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K