4GH2
Crystal Structure of the CHK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.145, 65.811, 57.993 |
| Unit cell angles | 90.00, 94.92, 90.00 |
Refinement procedure
| Resolution | 44.980 - 2.030 |
| R-factor | 0.1653 |
| Rwork | 0.164 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ot3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT (BUSTER 2.11.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 4.350 | 2.020 |
| Rmerge | 0.056 | 0.038 | 0.190 |
| Number of reflections | 21719 | ||
| <I/σ(I)> | 13.5 | ||
| Completeness [%] | 96.5 | 97.9 | 66.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 8000, Isopropanol, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
