4GGK
Crystal structure of Zucchini from mouse (mZuc / PLD6 / MitoPLD) bound to tungstate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.2716, 1.2983 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 38.859, 38.859, 213.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.270 - 2.100 |
R-factor | 0.21144 |
Rwork | 0.209 |
R-free | 0.25123 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1byr |
RMSD bond length | 0.013 |
RMSD bond angle | 1.382 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 10188 | |
<I/σ(I)> | 18.3 | 5.6 |
Completeness [%] | 99.5 | 98.1 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 50 mM Bis-Tris, pH 6.5, 18% PEG-3350, 2% tascimate, pH 6.0, 1:100 m/m ratio protein:chymotrypsin, VAPOR DIFFUSION, HANGING DROP, temperature 277K |