4GGC
Structural Analysis of Human Cdc20 Supports Multi-site Degron Recognition by APC/C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.281, 86.933, 48.339 |
| Unit cell angles | 90.00, 112.97, 90.00 |
Refinement procedure
| Resolution | 28.117 - 1.350 |
| R-factor | 0.133 |
| Rwork | 0.132 |
| R-free | 0.14640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.280 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.117 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.045 | 0.285 |
| Number of reflections | 65641 | |
| <I/σ(I)> | 23.5 | |
| Completeness [%] | 95.4 | 63.4 |
| Redundancy | 3.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES, 15% (w/v) PEG 6000, and 5% MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
