4GFG
Crystal structure of spleen tyrosine kinase complexed with r9021
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-21 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.061, 85.647, 90.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.470 - 2.350 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.24600 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3fqe |
RMSD bond length | 0.006 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.000 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 13618 | |
<I/σ(I)> | 16.6 | 4.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.3 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 278 | 12% PEG 3350, 0.1M sodium malonate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |