4GDF
A Crystal Structure of SV40 Large T Antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-08-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.99970 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.633, 128.307, 166.120 |
| Unit cell angles | 90.00, 89.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.227 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 70174 | |
| Completeness [%] | 93.2 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 291 | 100 mM bis-Tris (pH 6.75), 20% (v/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
