4GC9

Crystal structure of murine TFB1M in complex with SAM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	I 2 2 2
Unit cell lengths	47.700, 101.680, 211.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.467 - 2.103
R-factor	0.2067
Rwork	0.205
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gc5
RMSD bond length	0.003
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.103
Number of reflections	23216

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	2.9 M Sodium Acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K