4GAZ
Crystal Structure of a Jumonji Domain-containing Protein JMJD5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-19 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 69.431, 69.431, 270.052 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.180 - 2.810 |
R-factor | 0.2239 |
Rwork | 0.221 |
R-free | 0.27420 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.187 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.181 | 2.900 |
High resolution limit [Å] | 2.806 | 2.800 |
Number of reflections | 18410 | |
Completeness [%] | 94.8 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 289 | 100mM HEPES, 9% PEG 3350 (w/v), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |